La plateforme Lipidomique

The lipidomic platform is dedicated to broad spectrum analysis of apolar metabolites in biological matrices (biofluids, faeces, cells and tissues) to determine the molecular determinants of cardiometabolic diseases.

In order to cover a broad range of apolar metabolites, from very hydrophobic to more hydrophilic lipids, the lipidomic core utilizes two kinds of complementary instrumentations: LC/MS-MS for the determination of the phospho- and sphingolipidome and GC/MS for the determination of sterols as well as free fatty acids in biological matrices.

The core aims to develop in a near future analytical methodology for the determination of lyso phosphor- and sphingolipids as well as neutral lipids such as triglycerides, diglycerides and cholesteryl esters.

Equipment :

Prominence UFLC (Shimadzu) / 4000 QTrap (ABSciex)

 

Qtrap4000


4000 QTrap is ideal for a broad range of applications in clinical research:

 


•   Hybrid triple quadrupole/linear ion trap
•   Refrigerated autosampler of 70 positions

 

•   Enable high sensitivity, specificity with full scan MS, MS/MS et MS3
•   Perform Multiple Reaction Monitoring (MRM) scans for quantitation
•   Identification and validation of new biomarkers

 

Targeted analysis performed:
•    Phospholipids: PC, PE, PI, PS, PG, PA
•    Lyso phospholipids: LPC, LPE
•    Sphingolipids: SM, ceramides

GC-MS :
Trace 1310 - ISQ LT (Thermo Fisher Scientific)

GC-MS is ideal for a broad range of applications in clinical research:
•    Refrigerated autosampler of over 100 positions
•    Liquid, headspace or SPME injection
•    High acquisition rates compatible with fast GC
•   EI/CI+/CI- ionisation modes
•    AutoSIM and timed acquisition (tSIM)
•    High sensitivity

Targeted analysis performed :
•    Plasmatic sterols in mice and human
•    Fecal sterols in mice and human
•   Plasmatic free fatty acids

•    Sugar alcohols (mannitol/lactitol) in urine

 

 

STAFF


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Organigramme

  • Immeuble Ican
  • Immeuble Ican2
  • Immeuble Ican3
  • Ican Accueil
  • Immeuble Ican2
  • Immeuble Ican3
  • Ican Accueil
  • Immeuble Ican
  • Immeuble Ican3
  • Ican Accueil
  • Immeuble Ican
  • Immeuble Ican2